Growth of Russian Spring Summer Encephalitis (rsse) Virus in Monolayer Cell Cultures of Porcine Kidney, Bovine Embryo Kidney and Chicken Embryo.

Evaluation of the Embryonic Pathological Lesions and Efficacy of Amantadine against H9N2 Influenza Virus Using Chicken Embryo Model

Background & Aims: Various antiviral drugs such as amantadine are used to treat influenza. This drug is categorized in group C and few researches have been conducted about its toxic effects on human fetus. In the current study, the pathologic effects of the drug as well as drug efficacy in reducing influenza virus titer in the developing chicken embryo were assessed. <br ...

Comparision of different cell cultures for replication of infectious laryngotracheitis virus from chickens

The propagation of infectious laryngotracheitis virus (ILTV) has been described using primary cell cultures derived from chicken embryo liver and kidney or embryonated eggs, but these cultures use Specific Pathogen Free (SPF) eggs that are time and cost expensive. Since cell line cultures are easier to maintain in laboratory conditions, the growth of ILTV was evaluated in five different cell cu...

Growth of African Horse Sickness Virus in Monkey Kidney Cell Cultures

Development and Cytogenetic Characterization of a Continuous Bovine Kidney Cell Line (IRKHBK) and Evaluation its Susceptibility to some Viruses

In this syudy a continuous bovine kidney cell line derived from a primary bovine kidney cells was established for the first time in Iran. The cells were originating from two-day-old normal male calf of Holstein breed. The cell cultures were continuously passaged following complete proliferation of primary cells. The specific properties or characteristics of the cell were defined using cytogenet...

Naked DNA vaccines expressing the prM and E genes of Russian spring summer encephalitis virus and Central European encephalitis virus protect mice from homologous and heterologous challenge.

Naked DNA vaccines expressing the prM and E genes of two tick-borne flaviviruses, Russian spring summer encephalitis (RSSE) virus and Central European encephalitis (CEE) virus were evaluated in mice. The vaccines were administered by particle bombardment of DNA-coated gold beads by Accell gene gun inoculation. Two immunizations of 0.5 to 1 microg of RSSE or CEE constructs/dose, delivered at 4-w...